调节器
转录因子
RNA序列
计算生物学
抄写(语言学)
生物
一般转录因子
遗传学
编码
响应元素
发起人
基因
基因表达
转录组
语言学
哲学
标识
DOI:10.1007/978-3-319-23603-2_7
摘要
AbstractBacterial genomes encode numerous transcription factors, DNA-binding proteins that regulate transcription initiation. Identifying the regulatory targets of transcription factors is a major challenge of systems biology. Here I describe the use of two genome-scale approaches, ChIP-seq and RNA-seq, that are used to map transcription factor regulons. ChIP-seq maps the association of transcription factors with DNA, and RNA-seq determines changes in RNA levels associated with transcription factor perturbation. I discuss the strengths and weaknesses of these and related approaches, and I describe how ChIP-seq and RNA-seq can be combined to map individual transcription factor regulons and entire regulatory networks.KeywordsRegulatory networkTranscription factorMicroarrayTranscription profilingRNA-seqChromatin immunoprecipitationChIP-chipChIP-seq
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