The liquid culture media of cordyceps sinensis mycelia was optimized by means of orthogonal analysis.The results showed that the optimal medium for the mycelia was 20% potato,0.08% beef extraxt 0.2% peptone,0.15 % KH2PO4,0.15 % MgSO4·7H2O,1.5% sucrose,2.5% glucose and pH natural.In the cultural condition of 23 ℃,130r/min and 4 d,the mycelia biomass could gain 19.5 g/ L,which was as 2 times higher than that before optimization.