甘油三酯
化学
色谱法
稳定同位素比值
生物化学
胆固醇
量子力学
物理
作者
Xiaofang Li,Elizabeth J. Parks,David G. McLaren,Jessica E. Lambert,Helene L. Cardasis,Derek L Chappell,Thomas McAvoy,Gino Salituro,Achilles Alon,Justin Dennie,Manu V. Chakravarthy,Sudha Shankar,Omar Laterza,Michael E. Lassman
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2016-06-01
卷期号:8 (12): 1265-1277
被引量:3
标识
DOI:10.4155/bio-2016-0024
摘要
A traditional oral fatty acid challenge assesses absorption of triacylglycerol (TG) into the periphery through the intestines, but cannot distinguish the composition or source of fatty acid in the TG. Stable isotope-labeled tracers combined with LC-MRM can be used to identify and distinguish TG synthesized with dietary and stored fatty acids.Concentrations of three abundant TGs (52:2, 54:3 and 54:4) were monitored for incorporation of one or two (2)H11-oleate molecules per TG. This method was subjected to routine assay validation and meets typical requirements for an assay to be used to support clinical studies.Calculations for the fractional appearance rate of TG in plasma are presented along with the intracellular enterocyte precursor pool for 12 study participants.
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