葡萄糖氧化酶
化学
酶
过氧化物酶
磁性纳米粒子
聚合物
纳米颗粒
组合化学
纳米技术
化学工程
生物化学
材料科学
有机化学
工程类
作者
Hao Shen,Jiayi Song,Zixin Zhou,Mengqi Li,Ruiqi Zhang,Ping Su,Yi Yang
标识
DOI:10.1021/acs.iecr.9b01341
摘要
Immobilizing enzymes is an effective method to construct a multienzyme system for tandem reactions, but the anchored enzymes are too fragile for industrial applications. Here, an enzyme-shielding strategy is described to manufacture a multienzyme system with an “anchor-shield” structure. This system applies the self-assembly of supramolecular nucleotide coordinated polymers (NCPs) of controllable thickness encapsulating glucose oxidase (GOx) which is fixed on magnetic nanoparticles by DNA-directed immobilization. The nanocoating completely shields GOx, protecting it from denaturation and detachment. As a result, the stability of GOx against stresses was enhanced significantly; it maintained 83% and 64% of activity after incubation of 60 and 120 min under 60 °C. Notably, the encapsulated-enzyme retained 94% of activity after 45 days under 4 °C and 81% after 20 cycles, proving excellent reproducibility. More importantly, with the use of NCPs as a peroxidase-mimic, an enzyme–nanozyme cascade was established to be applicable for the removal of common pollutants, suggesting the potential of such magnetically separable systems.
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