Effect of methylnitronitrosoguanidine (MNNG) on the malignant transformation of human gastric mucosa GES-1 cells and its mechanism

Objective 　To investigate the effcts of methylnitronitrosoguanidine (MNNG) on the malignant transformation of human gastric mucosa GES-1 cells and explore the possible molecular mechanism. Methods 　The GES-1 cells was treated by 9 different concentrations of MNNG for 24h, then removed MNNG and culture cell normally. The cell proliferation was detected by CCK-8 assay after 24h. The effects of MNNG on invasion and migration ability were detected by Transwell invasion and migration tests after 24h. The apoptosis was detected by Annexin V-FITC/PI FCM after 48h, and the expression levels of NF-κB p65 mRNA and protein were measured by real-time fluorescent quantitative PCR and Western blotting respectively. Results 　2×10–4mol/L MNNG was chose for the induction of malignant transformation of GES -1 cells by the statistic analysis, 2×104mol/L MNNG could increase the proliferation, promote the invasion and migration, suppress the apoptosis of GES-1 cells (P<0.01). The expression levels of NF-κB p65 mRNA and protein were upregulated in GES-1 cells after treatment with MNNG (P<0.05). Conclusions 　MNNG can accelerate the proliferation, invasion and migration of GES-1 cells and inhibit apoptosis. This effect may be related to the activation of NF-κB. DOI: 10.11855/j.issn.0577-7402.2016.11.02