小球藻
沼渣
异养
生物量(生态学)
水解物
水解
食品科学
化学
藻类
铵
小球藻
厌氧消化
纤维素
生物化学
植物
农学
生物
有机化学
细菌
遗传学
甲烷
作者
Oskar Haske-Cornelius,Sonja Gierlinger,Robert Vielnascher,Wolfgang Gabauer,Katharina Prall,Alessandro Pellis,Georg M. Guebitz
标识
DOI:10.1016/j.algal.2021.102193
摘要
This study investigates the cellulases-catalyzed hydrolysis of newspaper waste and paper slurries as carbon source in combination with digestate from anaerobic digestion as nitrogen source for the heterotrophic cultivation of Chlorella sorokiniana. The enzymatic hydrolysis of paper slurries resulted in a hydrolysate containing 27.2 g l−1 glucose reflecting a 78% cellulose conversion. A maximum Chlorella sorokiniana biomass yield of 3.64 g l−1 was achieved within 91 h of cultivation under consumption of 9.7 g l−1 glucose. The most efficient cultivation converted 1 g l−1 glucose into 0.42 g l−1 biomass. A strong decrease in pH of the batch cultures due to ammonium consumption and CO2 production by algae cells inhibited full glucose consumption for high concentrations of 17 g l−1 glucose. Characterizing the amino acid content and pattern of the biomass revealed an amino acid fraction of 32.9% with Alanine as the most frequent amino acid.
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