Analysis of Three Flavonoids in Jujube Effervescent Granules by High performance liquid chromatography

Abstract In this research, high performance liquid chromatography (HPLC) was used to detect the content of rutin, myricetin and epicatechin in jujube effervescent granules. Methanol-0.1% formic acid water was used as the mobile phase, and the UV detection wavelength was 270 nm. The results showed that the content of rutin and myricetin has a good linear correlation (r>0.99) within the range of 1.0 μg to 50 μg, and the content of epicatechin also has a good linear correlation (r>0.99) in the range of 1.5 μg to 100 μg. The recovery of rutin, myricetin and epicatechin were 99.1 %, 98.6 % and 101.8 %, respectively. Furthermore, the total content of the three flavonoids in jujube effervescent granules was 472.19 μg/g, the content of rutin, myricetin and epicatechin was 433.75 μg/g, 6.46 μg/g and 31.98 μg/g, respectively.