氧化应激
姜黄素
脾脏
邻苯二甲酸二丁酯
维生素E
邻苯二甲酸盐
化学
炎症
药理学
谷胱甘肽
抗氧化剂
内分泌学
免疫学
医学
生物化学
酶
有机化学
作者
Xianliang Wang,Xu Yan,Yuyan Yang,Wenjing Yang,Yujing Zhang,Jiao Wang,Dan Ye,Yang Wu,Ping Ma,Biao Yan
标识
DOI:10.1016/j.fct.2019.110955
摘要
Dibutyl phthalate (DBP) is a ubiquitous environmental contaminant that at certain levels can be harmful to human health. Although DBP has been widely linked to immunotoxicity, any association between DBP exposure and splenic injury remains unknown. The purpose of this study was to investigate whether DBP exposure can induce splenic injury and the antagonistic effects of two antioxidants, vitamin E (VitE) and curcumin (Cur), on DBP-induced splenic injury. The levels of ROS, GSH, T-AOC, IL-1β, TNF-α, cytochrome C, caspase-8, caspase-9 and caspase-3 in the spleen homogenate of mice were measured. Any histopathological changes in the spleen were observed using H&E and toluidine blue staining. And the morphology of mitochondria was observed using Janus Green B staining. The results indicate that exposure to 50 mg/kg DBP could cause histopathological changes of the spleen and result in inflammation and apoptosis associated with oxidative stress, which may lead to splenic injury in mice. Moreover, both VitE and Cur could antagonize the oxidative stress induced by DBP to reduce splenic injury. These findings help to expand our understanding of DBP-mediated immunotoxicity, and to show that VitE and Cur can alleviate DBP-induced splenic injury and the possible DBP-associated decline in immune function.
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