单纯疱疹病毒
神经科学
顺行追踪
突触后电位
生物
轴浆运输
生物神经网络
病毒学
病毒
中枢神经系统
遗传学
受体
作者
Kathleen M. Fischer,Hannah K. Collins,Yan Pang,Dheeraj S. Roy,Ying Zhang,Guoping Feng,Shujing Li,Ádám Kepecs,Edward M. Callaway
摘要
Identification of synaptic partners is a fundamental task for systems neuroscience. To date, few reliable techniques exist for whole brain labeling of downstream synaptic partners in a cell-type-dependent and monosynaptic manner. Herein, we describe a novel monosynaptic anterograde tracing system based on the deletion of the gene UL6 from the genome of a cre-dependent version of the anterograde Herpes Simplex Virus 1 strain H129. Given that this knockout blocks viral genome packaging and thus viral spread, we reasoned that co-infection of a HSV H129 ΔUL6 virus with a recombinant adeno-associated virus expressing UL6 in a cre-dependent manner would result in monosynaptic spread from target cre-expressing neuronal populations. Application of this system to five nonreciprocal neural circuits resulted in labeling of neurons in expected projection areas. While some caveats may preclude certain applications, this system provides a reliable method to label postsynaptic partners in a brain-wide fashion.
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