胞浆
线粒体
体内
代谢物
细胞分离
生物化学
细胞器
分馏
生物
差速离心
离心
细胞生物学
细胞
化学
色谱法
酶
生物技术
作者
Fay M Allen,Ana S. H. Costa,Anja V. Gruszczyk,Georgina Bates,Hiran A. Prag,Efterpi Nikitopoulou,Carlo Viscomi,Christian Frezza,Andrew M. James,Michael P. Murphy
出处
期刊:FEBS Letters
[Wiley]
日期:2022-10-27
卷期号:597 (2): 246-261
被引量:1
标识
DOI:10.1002/1873-3468.14511
摘要
The compartmentation and distribution of metabolites between mitochondria and the rest of the cell is a key parameter of cell signalling and pathology. Here, we have developed a rapid fractionation procedure that enables us to take mouse heart and liver from in vivo and within ~ 30 s stabilise the distribution of metabolites between mitochondria and the cytosol by rapid cooling, homogenisation and dilution. This is followed by centrifugation of mitochondria through an oil layer to separate mitochondrial and cytosolic fractions for subsequent metabolic analysis. Using this procedure revealed the in vivo compartmentation of mitochondrial metabolites and will enable the assessment of the distribution of metabolites between the cytosol and mitochondria during a range of situations in vivo.
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