间充质干细胞
脐带
小RNA
细胞生物学
帘布衬里
干细胞
化学
分子生物学
生物
细胞分化
免疫学
成体干细胞
生物化学
基因
作者
Haoqing Yang,Wanqing Wang,Huina Liu,Chen Zhang,Yangyang Cao,Lujue Long,Xiao Han,Yuejun Wang,Fei Yan,Guoqing Li,Mengyuan Zhu,Luyuan Jin,Zhipeng Fan
摘要
Abstract To investigate the role and mechanism of FBLN1 in the osteogenic differentiation and bone regeneration by using umbilical cord mesenchymal stem cells (WJCMSCs). We found that FBLN1 promoted osteogenic differentiation of WJCMSCs and WJCMSC‐mediated bone regeneration. It was showed that there was an m 6 A methylation site in 3′UTR of FBLN1 mRNA, and the mutation of the m 6 A site enhanced the stability of FBLN1 mRNA, subsequently fostering the FBLN1 enhanced osteogenic differentiation of WJCMSCs. YTHDF2 was identified as capable of recognizing and binding to the m 6 A site, consequently inducing FBLN1 instability and repressed the osteogenic differentiation of WJCMSCs. Meanwhile, miR‐615‐3p negatively regulated FBLN1 by binding FBLN1 3′UTR and inhibited the osteogenic differentiation of WJCMSCs and WJCMSC‐mediated bone regeneration. Then, we discovered miR‐615‐3p was found to regulate the functions of FBLN1 facilitated by YTHDF2 through an m 6 A‐miRNA regulation mechanism. We demonstrated that FBLN1 is critical for regulating the osteogenic differentiation potentials of WJCMSCs and have identified that miR615‐3p mediated the decay of FBLN1 mRNA which facilitated by m 6 A reading protein YTHDF2. This provided a novel m 6 A‐miRNA epigenetic regulatory pattern for MSC regulation and bone regeneration.
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