The mass manufacture and extensive usage of plastics have brought about the spread of microplastics throughout the whole environment and even into the bodies of humans and animals. The toxic effects of microplastics cannot be ignored and their accumulation in the body is potentially dangerous. Therefore, scientists have used different methods to observe the morphology of microplastics in the mammal body and to detect and evaluate the levels of microplastics in the body. As a result, the toxicological consequences of plastic particles on the body are well understood. This review summarized some common methods of detecting microplastics in mammals, especially humans, by reviewing the research on microplastics conducted by different research groups in recent years. and evaluated the characteristics, detection goals, and advantages and disadvantages of each method. This establishes a more comprehensive overview of current microplastic detection technologies and can create a theoretical basis for future analysis of microplastic dynamics.