生物传感器
西维因
检出限
化学
乙酰胆碱酯酶
共价键
氧化还原
硫堇
色谱法
电化学
电极
组合化学
有机化学
杀虫剂
酶
生物化学
物理化学
生物
农学
作者
Ying Luo,Na Wu,Linyu Wang,Yonghai Song,Yan Du,Guangran Ma
出处
期刊:Biosensors
[MDPI AG]
日期:2022-08-10
卷期号:12 (8): 625-625
被引量:6
摘要
A ratiometric electrochemical biosensor based on a covalent organic framework (COFThi-TFPB) loaded with acetylcholinesterase (AChE) was developed. First, an electroactive COFThi-TFPB with a two-dimensional sheet structure, positive charge and a pair of inert redox peaks was synthesized via a dehydration condensation reaction between positively charged thionine (Thi) and 1,3,5-triformylphenylbenzene (TFPB). The immobilization of AChE on the positively charged electrode surface was beneficial for maintaining its bioactivity and achieving the best catalytic effect; therefore, the positively charged COFThi-TFPB was an appropriate support material for AChE. Furthermore, the COFThi-TFPB provided a stable internal reference signal for the constructed AChE inhibition-based electrochemical biosensor to eliminate various effects which were unrelated to the detection of carbaryl. The sensor had a linear range of 2.2–60 μM with a detection limit of 0.22 μM, and exhibited satisfactory reproducibility, stability and anti-interference ability for the detection of carbaryl. This work offers a possibility for the application of COF-based materials in the detection of low-level pesticide residues.
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