实验室烧瓶
振动器
细胞
细胞培养
选择(遗传算法)
产量(工程)
基因表达
悬浮培养
细胞生物学
生产力
生物
化学
基因
分子生物学
生物化学
计算机科学
材料科学
遗传学
人工智能
物理
宏观经济学
物理化学
量子力学
经济
冶金
振动
作者
Chuan Chen,Zening Wang,Zehua Sun,Wei Li,Dimiter S. Dimitrov
摘要
Compare with transient expression, stable cell lines generally have higher productivity and better quality for protein expression. However, selection of stable cell line is time-consuming and laborious. Here we describe an optimized selection method to achieve high-efficient stable cell pools with Expi293F suspension cells. This method only takes 2-3 weeks to generate stable cell pools with 2- to 10-fold higher productivity than transient gene expression (TGE). In fed-batch culture with Yeastolate, >1 g/L yield was achieved with our KTN0239-IgG stable cell pool in shaker flasks. This method can be also applied to efficiently display proteins on the cell surface.
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