烟草
部分
化学
嘧啶
微尺度热泳
立体化学
生物化学
基因
作者
Ningning Zan,Dandan Xie,Miao Li,Donghao Jiang,Baoan Song
标识
DOI:10.1021/acs.jafc.0c00987
摘要
Novel pyrimidine sulfide derivatives containing a dithioacetal and strobilurin moiety were designed and synthesized. Their antiviral activities against tomato chlorosis virus (ToCV) were investigated through the tomato chlorosis virus coat protein (ToCVCP)-oriented screening method. Microscale thermophoresis was used to study the interaction between the compound and the ToCVCP. Compounds B13 and B23 interacted better with ToCVCP than the other compounds and had dissociation constant (Kd) values of 0.09 and 0.06 μM, respectively. These values were lower than those of the control agents, ningnanmycin (0.19 μM) and ribavirin (6.54 μM), which indicated that the compounds had a strong binding effect with ToCVCP. Quantitative real-time polymerase chain reaction was used to evaluate the role of compounds B13 and B23 in the gene regulation of ToCVCP. Both compounds significantly reduced the expression level of the ToCVCP gene in Nicotiana benthamiana with reduction values of 88 and 83%, which were better than those of ningnanmycin (65%) and lead compound C14 (73%). Pyrimidine sulfide containing a dithioacetal and strobilurin moiety is significant in the research and development of novel anti-ToCV agents.
科研通智能强力驱动
Strongly Powered by AbleSci AI