3D生物打印
明胶
活力测定
自愈水凝胶
化学
移植
细胞存活
材料科学
组织工程
生物医学工程
细胞
生物化学
高分子化学
体外
外科
医学
作者
Ahmet Erdem,Mohammad Ali Darabi,Rohollah Nasiri,Sivakoti Sangabathuni,Yavuz Nuri Ertaş,Halima Alem,Vahid Hosseini,Amir Shamloo,Ali S Nasr,Samad Ahadian,Mehmet R. Dokmeci,Ali Khademhosseini,Nureddin Ashammakhi
标识
DOI:10.1002/adhm.201901794
摘要
Abstract Cell survival during the early stages of transplantation and before new blood vessels formation is a major challenge in translational applications of 3D bioprinted tissues. Supplementing oxygen (O 2 ) to transplanted cells via an O 2 generating source such as calcium peroxide (CPO) is an attractive approach to ensure cell viability. Calcium peroxide also produces calcium hydroxide that reduces the viscosity of bioinks, which is a limiting factor for bioprinting. Therefore, adapting this solution into 3D bioprinting is of significant importance. In this study, a gelatin methacryloyl (GelMA) bioink that is optimized in terms of pH and viscosity is developed. The improved rheological properties lead to the production of a robust bioink suitable for 3D bioprinting and controlled O 2 release. In addition, O 2 release, bioprinting conditions, and mechanical performance of hydrogels having different CPO concentrations are characterized. As a proof of concept study, fibroblasts and cardiomyocytes are bioprinted using CPO containing GelMA bioink. Viability and metabolic activity of printed cells are checked after 7 days of culture under hypoxic condition. The results show that the addition of CPO improves the metabolic activity and viability of cells in bioprinted constructs under hypoxic condition.
科研通智能强力驱动
Strongly Powered by AbleSci AI