Liquid Chromatography/Triple Quadrupole Tandem Mass Spectrometric Determination of Chloramphenicol Residue in Honey

A LC-MS/MS method was developed for the detection of chloramphenicol residue in honey. Samples were extracted using ethyl acetate, and the extracts were separated on a ZORBAX XDB-C18 column (2.1 mm×150 mm, 5 μm) with a mobile phase composed of methanol and water (40:60, V/V). The analysis was carried out by MS/MS in an electrospray negative ion multiple reaction monitoring (MRM) mode. The limits of detection and quantification were 0.1 ng/ml and 0.1 g/kg, respectively. The linear range was between 0.1 ng/ml and 10.0 ng/ml. The average spike recovery was 88.2%, with an average RSD of 3.26%.