OBJECTIVE: To study the toxic effects of pyrogallol on aquatic organisms. METHODS: The acute toxicities of different concentrations of pyrogallol on Chlorella pyrenoidosa, Scenedesmus obliquus and Moina macrocopa were studied. RESULTS: The algal cell densities and the quantity of the Moina macrocopa reduced significantly with pyrogallol concentration increased, exhibiting a good concentration-effects relationship. The EC50 of pyrogallol to Chlorella pyrenoidosa and Scenedesmus obliquus were 19.7 and 19.8 mg/L, respectively. The 28h and 48h EC50 of pyrogallol to Moina macrocopa were 18.25 and 13.96 mg/L, respectively. CONCLUSION: Pyrogallol had moderate toxicities on Chlorella pyrenoidosa, Scenedesmus obliquus and Moina macrocopa.