Influence of Danhongtongjing prescription on cytokines levels of blood and prostate tissue in autoimmune chronic prostatitis rats

Objective:To observe the influence of Danhongtongjing prescription on cytokines levels of blood and prostate tissue in autoimmune chronic prostatitis rats.Methods: 40 male Wistar rats which were 8-10 weeks old were randomly divided into blank control group(n=10),low dose group experiment group(n=10),medium dose experiment group(n=10) and high dose experiment group(n=10).The rat models were induced by using the prostate protein purified.The blank control group was given saline by intragastric administration,and the experiment groups were given low,medium and high dose of Danhongtongjing prescription suspension by intragastric administration,respectively.The course of treatment was 35 days.The carotid artery and prostate tissue of rats were collected.The expression of tumor necrosis factor-α(TNF-α) and interleukin-10(IL-10) in rats serum were quantitative analysed by double antibody sandwich enzyme immunosorbent assay(ELISA).The expression of TNF-α and IL-10 mRNA in rat prostate tissue were detected by real-time fluorescence quantitative PCR.Results: After the intervention,the TNF-α and IL-10 contents in rats serum of the control group and the each experiment group(low,medium and high dose) were(20.18±9.12) and(86.78±30.25)ng/L,(23.89±8.07) and(94.99±35.02)ng/L,(111.46±6.71) and(130.17±43.61)ng/L,(138.52±5.75) and(195.22±56.34)ng/L,respectively.The TNF-α,IL-10 contents in prostate tissue of the control group and the each experiment group were(0.17±0.02) and(0.58±0.11)ng/L,(0.27±0.02) and(0.81±0.03)ng/L,(0.35±0.11) and(0.93±0.19)ng/L,(0.98±0.03) and(0.99±0.34)ng/L,respectively.The differences had statistical significance,especially the differences in medium dose experiment group and high dose experiment group(P0.05 or P0.01).Conclusion: Danhongtongjing prescription can enhance the levels of TNF-α,IL-1 in blood and prostate tissue of rats which are induced by prostate protein purification.The effect is more obvious in medium dose experiment group and high dose experiment group compared with in low dose experiment group.