Objective: To compare the content of arctiin changes between decoction pieces of Fructus arctii and Fructus arctii powder in the process of decoction. Methods: The HPLC method was used for the determination of the content of arctiin changes in decoction of Fructus arctii and Fructus arctii powder at various decoction time. Results: The content of arctiin and decocted ratio in Fructus arctii powder are both greatly more than in decoction pieces of Fructus arctii within 60 minutes, and it had significant difference (P0.01). Conclusion: Chinese medicine decoction made from powder can save material and decoction time were proved, which provides application form of traditional Chinese medicine with innovative ideas.