Determination of Catalytic Activity for Peroxidase in the Oxidation-Polymerzation of Anline

Peroxidase catalyzes the oxidation polymerization of aniline.The catalytic activity peroxidase is measured by spectrophotometer. Appropriate conditions on examining the enzyme activity are studied, including appropriate substrate,enzyme concentration, substrate concentration,H 2O 2 concentration, temperature and pH value.Repeated measurements are carried out to confirm the accuracy of this method.