Kxpression of IRP2 mRNA in rat intestine with iron deficiency

Objective To explore the expression of IRP2 mRNA and it's regulating function in intestinal iron metabolism Methods Forty healthy adult Wistar rats were randomly divided into control group (n=10)and iron deficiency group(n=30).The model rats with iron deficiency were established. Serum iron(SI) was measured by flame assay and sFu was measured by radioimmunoassay.The total RNA of the duodenum membrane of rat was extracted hy the kit test.The expressions of IRP2 mRNA in duodenum in different anemia were tested by RT-PCR.Results The expression of IRP2 mRNA in control group was 0 .38 ± 0 .12 ,the group of recessive iron deficiency was 0.39±0.16, the group of mild tron deficiency anemia was 0.51±0. 08 , the group of moderate iron deficiency anemia was 0 .5 ± 0 .14.There was no difference between recessive iron deficiency group and control group(P0.05).The expres-sions of IRP2 mRNA in mild iron deficiency anemia group and modetate iron deficiency anemia group were significantly higher than those of control group(P0.05).In both recessive iron deficieney gronp and moderate iron deficiency anemia group, there was no dif-ference with mild iron deficiency anemia group(P0. 05). There was significant difference between recessive iron deficiency and mod-erate iron deficiency anemia group(P0.05).The expression of IRP2 mRNA increased as iron deficieney inereased.Conclusion IRP2 is an important regulatory protein in iron metabohsm.