Establishment of a two-step hyperinsulinemic euglycemic clamp technique

Objective To establish a two-step hyperinsulinemic euglycemic clamp technique. Methods A two-step hyperinsulinemic euglycemic clamp (30 mU·m~(-2)·min~(-1) and 120 mU·m~(-2)·min~(-1)) was established and applied to 10 nonobese healthy Chinese women with normal glucose (tolerance.) Results (1) During the clamp, the steady-state plasma insulin levels at the first step (60~90min) and the second step (150~180min) were(45.6±7.3)mIU/L and (217.8±27.2)mIU/L (respectively.) The steady-state glucose metabolism rates of the first step and the second step were (7.24±1.38) mg·kg~(-1)·min~(-1) and (13.08±1.16) mg·kg~(-1)·min~(-1) respectively. (2) During the two-step euglycemic clamp, there were significant decreases of serum cortisol, glucagon and free fatty acids compared with basal values while no significant changes occurred in serum growth hormone and C-peptide. Conclusion A two-step hyperinsulinemic euglycemic clamp was successfully established. During the clamp, the plasma insulin of high concentration inhibits lipid oxidation and the secretion of glucagon and cortisol.