木质纤维素生物量
生物量(生态学)
木聚糖酶
瘤胃
化学
生物技术
制浆造纸工业
基因组
碱金属
废物管理
生化工程
食品科学
生物燃料
生物
生物化学
酶
农学
发酵
工程类
有机化学
基因
作者
Shohreh Ariaeenejad,Mohsen Maleki,Elaheh Hosseini,Kaveh Kavousi,Ali Akbar Moosavi-Movahedi
标识
DOI:10.1016/j.biortech.2019.02.059
摘要
The aim of this study was to isolate and characterize novel alkali-thermostable xylanase genes from the mixed genome DNA of camel rumen metagenome. In this study, a five-stage computational screening procedure was utilized to find the primary candidate enzyme with superior properties from the camel rumen metagenome. This enzyme was subjected to cloning, purification, and structural and functional characterization. It showed high thermal stability, high activity in a broad range of pH (6–11) and temperature (30–90 °C) and effectivity in recalcitrant lignocellulosic biomass degradation. Our results demonstrated the power of in silico analysis to discover novel alkali-thermostable xylanases, effective for the bioconversion of lignocellulosic biomass.
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