纳米探针
转铁蛋白受体
铁蛋白
分子成像
体内
荧光寿命成像显微镜
病理
自体荧光
癌症
免疫组织化学
荧光
转铁蛋白
化学
癌症研究
医学
纳米颗粒
生物
材料科学
内科学
纳米技术
生物技术
物理
量子力学
作者
Yang Du,Kelong Fan,Hejun Zhang,Li Li,Peixia Wang,Jianxun He,Shigang Ding,Xiyun Yan,Jie Tian
标识
DOI:10.1016/j.nano.2018.07.007
摘要
Optical imaging technologies improve clinical diagnostic accuracy of early gastric cancer (EGC). However, there was a lack of imaging agents exhibiting molecular specificity for EGCs. Here, we employed the dye labeled human heavy-chain ferritin (HFn) as imaging nanoprobe, which recognizes tumor biomarker transferrin receptor 1 (TfR1), to enable specific EGC imaging using confocal laser endomicroscopy (CLE). TfR1 expression was initially examined in vitro in gastric tumor cells and in vivo through whole-body fluorescence and CLE imaging in tumor-bearing mice. Subsequently, dye labeled HFn was topically applied to resected human tissues for EGC detection. CLE analysis of TfR1-targeted fluorescence imaging allowed distinction of neoplastic from non-neoplastic tissues (P < 0.0001), and TfR1 expression level was found to correlate with EGC differentiation degrees (P < 0.0001). Notably, the CLE evaluation correlated well with the immunohistochemical findings (κ-coefficient: 0.8023). Our HFn-nanoprobe-based CLE increases the accuracy of EGC detection and enables visualization of tumor margins and endoscopic resection.
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