苦参
化学
丙炔基转移酶
预酸化
类黄酮
立体专一性
黄烷酮
黄酮类
立体化学
生物化学
酶
催化作用
色谱法
苦参碱
抗氧化剂
作者
Ridao Chen,Xiao Liu,Jianhua Zou,Yunze Yin,Bin Ou,Jianhua Li,Ruishan Wang,Dan Xie,Pei‐Cheng Zhang,Jungui Dai
标识
DOI:10.1002/adsc.201300196
摘要
Abstract Prenylflavonoids are valuable natural products that are widely distributed in plants. They often possess divergent biological properties, including phytoestrogenic, anti‐bacterial, anti‐tumor, and anti‐diabetic activities. The reaction catalyzed by prenyltransferases represents a Friedel–Crafts alkylation of the flavonoid skeleton in the biosynthesis of natural prenylflavonoids and often contributes to the structural diversity and biological activity of these compounds. However, only a few plant flavonoid prenyltransferases have been identified thus far, and these prenyltransferases exhibit strict substrate specificity and low catalytic efficiency. In this article, a flavonoid prenyltransferase from Sophora flavescens , SfFPT, has been identified that displays high catalytic efficiency with high regiospecificity acting on C‐8 of structurally different types of flavonoid (i.e., flavanone, flavone, flavanonol, and dihydrochalcone, etc.). Furthermore, SfPFT exhibits strict stereospecificity for levorotatory flavanones to produce (2 S )‐prenylflavanones. This study is the first to demonstrate the substrate promiscuity and stereospecificity of a plant flavonoid prenyltransferase in vitro . Given its substrate promiscuity and high catalytic efficiency, SfFPT can be used as an environmentally friendly and efficient biological catalyst for the regio‐ and stereospecific prenylation of flavonoids to produce bioactive compounds for potential therapeutic applications.
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