Optimization of 1,3‐propanediol production by novel recombinant Escherichia coli using response surface methodology

Abstract Response surface methodology was used to optimize 1,3‐propanediol production by a novel recombinant Escherichia coli JM109 (pHsh‐ dha B‐ yqh D). The optimal fermentation parameters for enhanced 1,3‐propanediol yield were found to be: glycerol 61.8 g L −1 , yeast extract 6.2 g L −1 , Vitamin B 12 0.049 g L −1 and fermentation time 30 h. Subsequent experimental trials confirmed the validity of the model. These optimal fermentation conditions in the cultivation flask culture led to a 1,3‐propanediol concentration of 43.1 g L −1 and a conversion rate of 69.7% (g g −1 ). A maximum 1,3‐propanediol concentration of 41.1 g L −1 was achieved in a 5 L fermenter using the optimized parameters. Copyright © 2006 Society of Chemical Industry