化学
电化学发光
适体
MUC1号
组合化学
胶体金
猝灭(荧光)
纳米颗粒
纳米技术
光化学
粘蛋白
荧光
检出限
色谱法
分子生物学
生物化学
量子力学
生物
物理
材料科学
作者
Xinya Jiang,Huijun Wang,Haijun Wang,Ruo Yuan,Yaqin Chai
标识
DOI:10.1021/acs.analchem.6b02501
摘要
In the present work, we first found that mercury ion (Hg2+) has an efficient quenching effect on the electrochemiluminescence (ECL) of N-(aminobutyl)-N-(ethylisoluminol) (ABEI). Since we were inspired by this discovery, an aptamer-based ECL sensor was fabricated based on a Hg2+ triggered signal switch coupled with an exonuclease I (Exo I)-stimulated target recycling amplification strategy for ultrasensitive determination of Hg2+ and mucin 1 (MUC1). Concretely, the ECL intensity of ABEI-functionalized silver nanoparticles decorated graphene oxide nanocomposite (GO-AgNPs-ABEI) was initially enhanced by ferrocene labeled ssDNA (Fc-S1) (first signal switch “on” state) in the existence of H2O2. With the aid of aptamer, assistant ssDNA (S2) and full thymine (T) bases ssDNA (S3) modified Au nanoparticles (AuNPs-S2-S3) were immobilized on the sensing surface through the hybridization reaction. Then, via the strong and stable T-Hg2+-T interaction, an abundance of Hg2+ was successfully captured on the AuNPs-S2-S3 and effectively inhibited the ECL reaction of ABEI (signal switch “off” state). Finally, the signal switch “on” state was executed by utilizing MUC1 as an aptamer-specific target to bind aptamer, leading to the large decrease of the captured Hg2+. To further improve the sensitivity of the aptasensor, Exo I was implemented to digest the binded aptamer, which resulted in the release of MUC1 for achieving target recycling with strong detectable ECL signal even in a low level of MUC1. By integrating the quenching effect of Hg2+ to reduce the background signal and target recycling for signal amplification, this proposed ECL aptasensor was successfully used to detect Hg2+ and MUC1 sensitively with a wide linear response.
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