浊度
色谱法
光散射
粒径
相(物质)
化学
稀释
分析化学(期刊)
散射
物理
光学
生物
热力学
生态学
物理化学
有机化学
作者
Yanan Huang,Yinge Bai,Wenhan Jin,Di Shen,Haochen Lyu,Lianggang Zeng,Mengdie Wang,Yu Liu
出处
期刊:Biochemistry
[American Chemical Society]
日期:2021-08-09
卷期号:60 (32): 2447-2456
被引量:4
标识
DOI:10.1021/acs.biochem.1c00386
摘要
The turbidity assay is commonly exploited to study protein liquid-to-liquid phase separation (LLPS) or liquid-to-solid phase separation (LSPS) processes in biochemical analyses. Herein, we present common pitfalls of this assay caused by exceeding the detection linear range. We showed that aggregated proteins of high concentration and large particle size can lead to inaccurate quantification in multiple applications, including the optical density measurement, the thermal shift assay, and the dynamic light scattering experiment. Finally, we demonstrated that a simple sample dilution of insoluble aggregated protein (LSPS) samples or direct imaging of liquid droplets (LLPS) can address these issues and improve the accuracy of the turbidity assay.
科研通智能强力驱动
Strongly Powered by AbleSci AI