剪接体
RNA剪接
内含子
RNA聚合酶Ⅱ
抄写(语言学)
小剪接体
生物
核糖核蛋白
核糖核酸
遗传学
拼接因子
小核RNA
细胞生物学
外显子剪接增强剂
基因
RNA依赖性RNA聚合酶
发起人
基因表达
语言学
哲学
作者
Lydia Herzel,Diana S. M. Ottoz,Tara Alpert,Karla M. Neugebauer
摘要
Pre-mRNA splicing occurs on nascent RNA, which is attached to chromatin by RNA polymerase II. Much splicing occurs co-transcriptionally, and the spatial and temporal coordination of the two processes is tightly coordinated with other mRNA-processing events. Several macromolecular machines collaborate to produce eukaryotic messenger RNA. RNA polymerase II (Pol II) translocates along genes that are up to millions of base pairs in length and generates a flexible RNA copy of the DNA template. This nascent RNA harbours introns that are removed by the spliceosome, which is a megadalton ribonucleoprotein complex that positions the distant ends of the intron into its catalytic centre. Emerging evidence that the catalytic spliceosome is physically close to Pol II in vivo implies that transcription and splicing occur on similar timescales and that the transcription and splicing machineries may be spatially constrained. In this Review, we discuss aspects of spliceosome assembly, transcription elongation and other co-transcriptional events that allow the temporal coordination of co-transcriptional splicing.
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