核酶
四环素
变构调节
锤头状核酶
连接器
生物
基因
劈理(地质)
酵母
核糖核酸
计算生物学
遗传学
化学
细胞生物学
生物化学
酶
操作系统
抗生素
古生物学
计算机科学
断裂(地质)
作者
Alexander Wittmann,Beatrix Suess
出处
期刊:Molecular BioSystems
[The Royal Society of Chemistry]
日期:2011-01-01
卷期号:7 (8): 2419-2419
被引量:72
摘要
Synthetic regulatory devices are key components for the development of complex biological systems and the reprogramming of cellular functions and networks. Here we describe the selection of tetracycline inducible hammerhead ribozymes. A tetracycline aptamer was fused to the full-length hammerhead ribozymevia a variable linker region. 11 rounds of in vitro selection were applied to isolate linker sequences that mediate tetracycline dependent hammerhead cleavage. We identified allosteric ribozymes that cleave in the presence of 1 μM tetracycline as fast as the full-length ribozyme whereas cleavage is inhibited up to 333-fold in the absence of tetracycline. Reporter gene assays indicate that the allosteric ribozymes can be employed to control gene expression in yeast.
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