[Objective] The research aimed to provide the basis for further development and utilization of apple pomace.[Method] Total flavonoids were extracted from apple pomace by using ultrahigh voltage equipment.The antibacterial test was made on total flavonoids extract from apple pomace at five concentrations(0.8,0.6,0.4,0.2 and 0.1 mg/ml).[Result] Total flavonoids from apple pomace had better antibacterial effects on Escherichia coli and Staphylococcus aureus.With the increasing of total flavonoids concentration,the antimicrobial action was also enhanced.[Conclusion] This method provided a new approach for the comprehensive utilization of apple pomace.