Effect of Gemcitabine on Proliferation,Apoptosis and PLK-1 Expression in Pancreatic Cancer Cells

Objective To study the effect of Gemcitabine on SW1990 cell proliferation,apoptosis and PLK-1 expression in pancreatic cancer. Methods Different concentrations of Gemcitabine( 0,1,2,5 and 10 μmol / L) were respectively added into human pancreatic cancer SW1990 cells,and the cell survival rates were detected after being cultured for 3 d. Conditions of SW1990 cells apoptosis treated by 0,2 and 5 μmol / L Gemcitabine,PLK-1 mRNA and protein expressions were detected. Results The differences in SW1990 survival and apoptosis rates of Gemcitabine for 24,48 and72 h of treatment by different concentrations of Gemcitabine were statistically significant compared with those by 0 μmol / L Gemcitabine( P 0. 05),and the SW1990 inhibition effect by Gemcitabine was more obvious with the increasing concentration. The PLK-1 mRNA and protein expression levels in 5 μmol / L group after 24,48 and 72 h treatment were significantly higher than those by 0 μmol / L Gemcitabine( P 0. 05). Conclusion Gemcitabine can effectively induce apoptosis of pancreatic cancer cells,and the mechanism is related to the SW1990 cell PLK-1 mRNA and protein expression levels.