Influence of calcitonin gene-related peptide on proliferation of vascular smooth muscle cells

Objective: To evaluate the effect of neuropeptide on the proliferation and migration of vascular smooth muscle cells(VSMCs).Methods: VSMCs obtained from human embrynic aorta were cultured in vitro.The experiment was divided into 3 groups:diamide group,CGRP group and diamide plus CGRP group.The proliferous change of VSMCs was confirmed by using the MTT and cell counter method.The mRNA expression change of the cyclin D1 and E was detected by reverse transcription-polymerase chain raction method(RT-PCR) and the changes of the cell cycle by flow cytometry.Results: CGRP promoted VSMC proliferation at the beginning.CGRP presented intensive inhibition on the proliferous VSMCs induced by diamide.CGRP down-regulated the mRNA expression of cyclin D1.Conclusion: The diamide can induce the VSMCs proliferation;CGRP can down-regulate the proliferation of VSMCs induced by diamide.CGRP might have regulative effect on the proliferation of VSMCs,but the mechanism need further study.