Detection of NDV Infection and Antigen Location by Indirect Immunocytochemistry

The objective is to establish a simple,convenient,accurate,specific method to detect the NDV and detect the location of NDV in the COS-7 by comparing the indirect HRP-immunocytochemistry and indirect fluorescence immunocytochemistry.Both two experiment methods can detect the location of NDV and it was in the cell plasm of the COS-7.The indirect HRP-immunocytochemistry request the dilution of the first antibody is less than 1∶100,but the indirect fluorescence immunocytochemistry request the dilution of the first antibody is less than 1∶100.The indirect HRP-immunocytochemistry request the dilution of the second antibody is less than 1∶300,but the indirect fluorescence immunocytochemistry request the dilution of the second antibody is less than 1∶50.Further more,when detect the results that the indirect HRP-immunocytochemistry is more convenient than the indirect fluorescence immunocytochemistry.The indirect HRP-immunocytochemistry is simple,convenient,accurate and specific method to detect the NDV.