Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice

ABSTRACT Objective Objective: To explore the effect of Compound Tufuling Granules (复方土茯苓颗粒, CTG) on : To explore the effect of Compound Tufuling Granules (复方土茯苓颗粒, CTG) on regulating glucose transporter 9 (GLUT9) expression in the kidney to influence the uric acid excretion by regulating glucose transporter 9 (GLUT9) expression in the kidney to influence the uric acid excretion by the kidney and serum uric acid (SUA) level in hyperuricemia mice. the kidney and serum uric acid (SUA) level in hyperuricemia mice. Methods Methods: Sixty Kunming male mice were : Sixty Kunming male mice were randomly divided into the control group, model group, benzbromarone group, and CTG high-, middle- and low- randomly divided into the control group, model group, benzbromarone group, and CTG high-, middle- and low- dose groups. The yeast extract and uricase inhibition method were used to build hyperuricemia model, and dose groups. The yeast extract and uricase inhibition method were used to build hyperuricemia model, and the corresponding drugs were administrated on the 7th day. On the 21st day the 24-h urine was collected, the corresponding drugs were administrated on the 7th day. On the 21st day the 24-h urine was collected, on the 22nd day the blood was collected, the SUA level was detected by uricase colorimetry, and the mRNA on the 22nd day the blood was collected, the SUA level was detected by uricase colorimetry, and the mRNA and protein expressions of GLUT9 were detected by quantitative real-time polymerase chain reaction and and protein expressions of GLUT9 were detected by quantitative real-time polymerase chain reaction and Western blot, respectively. Western blot, respectively. Results Results: Compared with the model group, the levels of SUA and the mRNA and : Compared with the model group, the levels of SUA and the mRNA and protein expressions of GLUT9 were signifi cantly decreased, and the fraction excretion of uric acid (FEUA) was protein expressions of GLUT9 were signifi cantly decreased, and the fraction excretion of uric acid (FEUA) was significantly increased in the CTG groups and benzbromarone group (all significantly increased in the CTG groups and benzbromarone group (all P 0.05). SUA >0.05). SUA is positively related to the GLUT9 mRNA and protein expressions in the kidney ( is positively related to the GLUT9 mRNA and protein expressions in the kidney (P<0.05 or <0.05 or P<0.01). <0.01). Conclusions Conclusions: CTG can signifi cantly reduce the SUA and increase the FEUA. In addition, CTG can effectively inhibit the mRNA CTG can signifi cantly reduce the SUA and increase the FEUA. In addition, CTG can effectively inhibit the mRNA and protein expressions of GLUT9 in the kidney of hyperuricemia mice to inhibit the uric acid re-absorption, and protein expressions of GLUT9 in the kidney of hyperuricemia mice to inhibit the uric acid re-absorption, promote uric acid excretion and reduce SUA. promote uric acid excretion and reduce SUA.