Study on preparation of small peptide of plant by compound enzyme hydrolysis

The optimum technology conditions of preparation of plant peptide with the mixture of soy protein and peanut protein microfluidized by compound protease(papain and trypsin) and flavourzyme hydrolysis were studied.The results showed that the degree of hydrolysis could increase 2%～3% after microfluidized.The optimum conditions of hydrolysis were as follows:the homogeneous pressure 60MPa,the composition proportion of papain and trypsin 1∶1,the hydrolysis conditions of the compound protease:temperature 60℃,pH6.5,enzyme 250U/g,substrate concentration 5%,those of flavourzyme:temperature 55℃,pH6.0,enzyme 200U/g.The molecular weight of the purified hydrolysate determined by HPGPC was mainly below 3000Da.