Immunoregulatory Effects of Compound Ammonium Glycyrrhizin Soluble Powder on Liver Injury Induced by Enrofloxacin and LPS in Chickens

【Objective】 The present study was aimed at the effects of compound ammonium glycyrrhizin soluble powder(CAG) on proinflammatory cytokines production and T-cell subsets deviation in peripheral blood in chicken liver injury induced by enrofloxacin and lipopolysaccharide crude extract(LPS).【Method】A total of 104 animals were allocated randomly into 5 groups named I-V: I normal group,II liver injury group(model group),III prevention group,IV high dosage treatment group and V low dosage treatment group.Group II-IV were administered with enrofloxacin(100 mg.kg-1) once a day for three days and on the third day administered with LPS(4 mL.kg-1) at the same time.Group III received prior administration of CAG at the dosage of 40 mg.L-1(by ammonium glycyrrhizin,the same as follows) in drinking water for three days before establishment of liver injury model.Groups IV and V received CAG at a dosage of 22.5 and 7.5 mg.kg-1 twice a day for three days after LPS treatment.Every eight chickens were sacrificed at 6,24 and 48 h following post-treatment with LPS and their blood samples were collected for detection.【Result】Compared with group I,the group II levels of serumALT,AST,TNF-α,IL-1 and IL-6 were significantly higher at 6,24,48 h post-treatment with LPS and CD3(+),CD4(+) and CD8(+) T-lymphocytes were significantly lower at 6 and 24 h higher at 48 h post-treatment with LPS.When compared with group II,there existed a significant fall in the levels of serum ALT,TNF-α,IL-6 at 6 h,AST at 6 h and 24 h and the number of CD3(+),CD4(+) and CD8(+) T-lymphocytes at 48 h in group III.In group IV,the levels of ALT,AST,TNF-α,IL-6 at 6 h and 24 h,IL-1 and CD3(+),CD4(+) and CD8(+) T-lymphocytes at 48 h decreased significantly and CD3(+) T-lymphocytes at 6 h and 24 h,CD4(+) T-lymphocytes at 24 h increased obviously.In group V,there was a significant fall in CD8(+) T-lymphocytes at 48 h and no obvious other differences were detected.The liver histopathological changes got a better improvement in group IV than others.【Conclusion】The novel liver injury induced by enrofloxacin and LPS effects might be related to TNF-α,IL-1 and IL-6 production and CD3(+),CD4(+) and CD8(+) T-cell deviation in peripheral blood.CAG prevents the liver injury through inhibiting TNF-α,IL-1 and IL-6 production and regulating the number of T-cell subsets in peripheral blood by bi-directional regulation and the efficacy might be related to dosage and treatment time.