Natural sepiolite was activated by acid-treatment under different conditions and used as the support. Several Au/sepiolite catalysts for selective catalytic reduction (SCR) of NOx by CO were prepared by impregnation, using Au(PPh_3)(NO_3) as the precursor. The optimum acid-treatment condition for sepiolite was determined by orthogonal experiments. Activity of Au/sepiolite for NO reduction was investigated. The catalyst samples were characterized by TGA, XRD, BET and IR. The results showed that Au/sepiolite catalysts exhibited satisfactory activity for SCR of NOx by CO.