Comparison study of the techniques for the predict of the blood perfusion of avulsion skin flap in pig

Objective To investigate the techniques for predict of the blood perfusion of the tissue in skin avulsion injury. Methods A machine was developed to mimic traffic accident and to create an avulsion injury pattern in hind leg of pig. A 4?cm×10?cm random avulsed skin flap was created in the avulsion area. Thermometer, laser Doppler blood flow-meter and fluorescein were adopted to measure the blood perfusion in avulsion skin flap. Results When surface temperature of the avulsion skin flap descended 2.4?℃, Doppler index was lower than 49.80%, the avulsed flap would become necrosis. The real survival area of avulsed skin flap was 8.51% less than what was predicted with fluorescein. There was secondary necrosis happened in avulsed skin flaps. The survival area of avulsed skin flap was 33.16% less than that of same size normal skin flap. Conclusion The fluorescein is a nice technique to predict blood perfusion if it was used with other techniques.