DNA Barcoding on the Identification of 8 Species of Raptors

The 648 bp mitochondrial cytochrome c oxidase subunit I(COI) gene was examined in 8 species of raptors(5 species in Accipitridae,Falconiformes;3 species in Strigidae,Strigiformes) by PCR with universal primers.99 additional barcode sequences were downloaded to explore whether the DNA barcodes could distinguish between these species.The result shows that in total 75.6% species are under the 10× rule,80.5% species possess unique DNA barcode sequences by which they could be identified.On the other hand,19.5% species(including 8 species of Buteo) are difficult to be distinguished by DNA barcoding.