色谱法
化学
液相色谱-质谱法
质谱法
电喷雾
衍生化
串联质谱法
电喷雾电离
萃取(化学)
液-液萃取
氯化丹酯
作者
Julie A. Ray,Mark M. Kushnir,Alan L. Rockwood,A.W. Meikle
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 99-108
被引量:6
标识
DOI:10.1007/978-1-4939-3182-8_12
摘要
We describe a direct method of measurement of free estradiol using equilibrium dialysis followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Serum aliquots and internal standards are extracted by liquid-liquid extraction using methyl-tert-butyl ether (MTBE) followed by derivatization with dansyl chloride. An API 5500 mass spectrometer operated in positive electrospray mode is used for detection.
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