PRC2
生物
多组蛋白
组蛋白
基因沉默
西斯特
组蛋白H2A
细胞生物学
抑制因子
遗传学
组蛋白H3
X-失活
基因
基因表达
X染色体
作者
Lígia Tavares,Emilia Dimitrova,David Oxley,Judith Webster,Raymond A. Poot,Jeroen Demmers,Karel Bezstarosti,Stephen Taylor,Hiroki Ura,Hiroshi Koide,Anton Wutz,Miguel Vidal,Sarah Elderkin,Neil Brockdorff
出处
期刊:Cell
[Elsevier]
日期:2012-02-01
卷期号:148 (4): 664-678
被引量:516
标识
DOI:10.1016/j.cell.2011.12.029
摘要
Polycomb-repressive complex 1 (PRC1) has a central role in the regulation of heritable gene silencing during differentiation and development. PRC1 recruitment is generally attributed to interaction of the chromodomain of the core protein Polycomb with trimethyl histone H3K27 (H3K27me3), catalyzed by a second complex, PRC2. Unexpectedly we find that RING1B, the catalytic subunit of PRC1, and associated monoubiquitylation of histone H2A are targeted to closely overlapping sites in wild-type and PRC2-deficient mouse embryonic stem cells (mESCs), demonstrating an H3K27me3-independent pathway for recruitment of PRC1 activity. We show that this pathway is mediated by RYBP-PRC1, a complex comprising catalytic subunits of PRC1 and the protein RYBP. RYBP-PRC1 is recruited to target loci in mESCs and is also involved in Xist RNA-mediated silencing, the latter suggesting a wider role in Polycomb silencing. We discuss the implications of these findings for understanding recruitment and function of Polycomb repressors.
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