球体
转分化
恶性肿瘤
导管细胞
3D生物打印
胰腺炎
胰腺
胰腺癌
癌症
腺泡细胞
生物医学工程
胰管
生物
病理
癌症研究
医学
内科学
细胞培养
干细胞
细胞生物学
组织工程
遗传学
作者
D. Hakobyan,Chantal Médina,Nathalie Dusserre,Marie‐Laure Stachowicz,Charles Handschin,Jean-Christophe Fricain,Julie Guillermet-Guibert,Hugo Oliveira
出处
期刊:Biofabrication
[IOP Publishing]
日期:2020-04-16
卷期号:12 (3): 035001-035001
被引量:56
标识
DOI:10.1088/1758-5090/ab7cb8
摘要
Pancreatic ductal adenocarcinoma (PDAC) is the most common malignancy of the pancreas. It has shown a poor prognosis and a rising incidence in the developed world. Other pathologies associated with this tissue include pancreatitis, a risk condition for pancreatic cancer. The onset of both pancreatitis and pancreatic cancer follows a common pattern: exocrine pancreatic acinar cells undergo a transdifferentiation to duct cells that triggers a 3D restructuration of the pancreatic tissue. However, the exact mechanism underlying this process remains partially undefined. Further understanding the cellular events leading to PDAC could open new avenues in the development of novel therapeutic approaches. Since current 2D cell culture models fail to mimic the tridimensional complexity of the pancreatic tissue, new in vitro models are urgently needed. Here, we generated 3D pancreatic cell spheroid arrays using laser-assisted bioprinting and characterized their phenotypic evolution over time through image analysis and phenotypic characterization. We show that these bioprinted spheroids, composed of both acinar and ductal cells, can replicate the initial stages of PDAC development. This bioprinted miniaturized spheroid-based array model should prove useful for the study of the internal and external factors that contribute to the formation of precursor PDAC lesions and to cancer progression, and may therefore shed light on future PDAC therapy strategies.
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