化学
乙醇沉淀
糖苷键
刚果红
多糖
阿拉伯糖
半乳糖
残留物(化学)
侧链
二维核磁共振波谱
单糖
甘露糖
立体化学
生物化学
色谱法
有机化学
发酵
聚合物
木糖
酶
吸附
作者
Paiheerding Mutaillifu,Х. М. Бобакулов,Aytursun Abuduwaili,Haibaier Huojiaaihemaiti,Rehebati Nuerxiati,Haji Akber Aisa,Abulimiti Yili
标识
DOI:10.1016/j.ijbiomac.2019.11.245
摘要
A water-soluble polysaccharide, named GPN, with molecular mass 38.7 kDa was isolated from Glycyrrhiza glabra with hot water extraction, ethanol precipitation, and purified by column chromatography. Monosaccharide composition analysis confirmed the presence of predominant glucose (98.03%) and trace amount of mannose, arabinose, and galactose. Methylation and GC-MS analysis revealed that the main glycosidic bonds in GPN comprised 1,4-linked Glcp, T-linked Glcp, 1,4,6-linked Glcp, and 1,6-linked Glcp. Based on these results and 1D/2D NMR spectroscopy, GPN has a linear backbone of 1,4-linked α-D-Glcp and 1,6-linked α-D-Glcp which substituted at C-4 of glucose. The side chain probably composed from 1,4-linked to main side α-D-Glcp and terminal 1-linked β-D-Glcp to the C-3 of D residue. Congo red assay confirmed the existence of triple helix structure. Moreover, SEM and XRD analysis revealed that the GPN had irregular fibrous, filaments like surface; and both crystalline and amorphous structure. GPN also displayed favorable thermal stability. Moreover, G.glabra polysaccharide showed good antioxidant activity.
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