A novel ratiometric fluorescence probe for highly sensitive and specific detection of chlorotetracycline among tetracycline antibiotics

It is of great importance to detect chlorotetracycline (CTC) in a highly sensitive and specific way because of its wide distribution in aquaculture and animal husbandry. Herein, we propose a novel ratiometric fluorescence strategy to assay CTC by using bovine serum albumin stabilized gold nanoclusters (BSA-AuNCs). The BSA-AuNCs consisting of 25 gold atoms (Au25NCs) display a red emission at 640 nm (λex = 370 nm). In the presence of CTC, a new blue emission at 425 nm is emerged and its intensity dramatically increases with the addition of more the analyte; meanwhile the red emission at 640 nm shows a linear decrease reversely. However, at identical conditions neither the analogues of CTC as tetracycline (TC), oxytetracycline (OTC) or doxycycline (DC) induces similar response of BSA-AuNCs. Such interesting phenomenon is proven related to the conversion from large Au25NCs to smaller nanoclusters composing 8 gold atoms (Au8NCs), which intrinsically originate from the interaction between CTC and the ligand BSA. Therefore, a ratiometric probe is established to sensitively detect CTC in the wide range (0.2-10 μM) with a low limit of detection (LOD) at 65 nM. In addition, this strategy can also be applied to assay CTC in human serum, showing great promise for practical applications in future.