表位
过敏原
消化(炼金术)
肽
化学
生物
生物化学
抗体
免疫学
过敏
色谱法
作者
Xin Guo,Songsong Jiang,Xinrui Li,Shuai Yang,Lei Cheng,Jinyu Qiu,Huilian Che
出处
期刊:Food Chemistry
[Elsevier]
日期:2020-08-01
卷期号:322: 126711-126711
被引量:6
标识
DOI:10.1016/j.foodchem.2020.126711
摘要
Jug r 1, the major allergen of walnut, triggers severe allergic reactions through epitopes. Hence, research on the efficient strategy for analyzing the linear epitopes of Jug r 1 are necessary. In this work, bioinformatics analysis was used to predict the linear epitopes of Jug r 1. Overlapping peptide synthesis was used to map linear epitopes. In vitro simulated gastrointestinal digestion and HPLC-MS/MS were used to identify digestion-resistant peptides. The results showed that six predicted linear epitopes were AA28-35, AA42-49, AA55-62, AA65-73, AA97-104, and AA109-121. AA16-30 and AA125-139 were identified by the sera of walnut allergic patients. Five digestion-resistant peptides were AA19-33, AA40-45, AA54-74, AA96-106, and AA117-137. The predicted results only included one of the linear epitopes identified by sera, while the digestion-resistant peptides covered all. Therefore, the digestion-resistant property of food allergens may be a promising direction for studying the linear epitopes of Jug r 1.
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