化学
适体
量子点
光电流
检出限
电极
生物分析
氧化铟锡
低聚物
纳米技术
光电子学
材料科学
色谱法
有机化学
物理化学
生物
遗传学
作者
Yuwei Bu,Mengjie Zhang,Junliang Fu,Xiaoyan Yang,Shufeng Li
标识
DOI:10.1016/j.aca.2021.339200
摘要
In this paper, a quantitative cathodic photoelectrochemical aptasensor is described by using black phosphorous quantum dots (BPQDs) as photoactive material and assisted by heme as electron acceptor for sensing of amyloid β peptide (Aβ). Specifically, BPQDs were synthesized by solvothermal method and characterized by various techniques. The as-prepared BPQDs were assembled on the transparent indium tin oxide electrode, and the positively charged poly-l-lysine (PLL) was then absorbed onto BPQDs via electronic interaction. Subsequently, the aptamer as the specific recognition element for Aβ oligomer was introduced on the BPQDs-PLL modified electrode. After bound with heme to form Aβ-heme complex, Aβ oligomer was simultaneously captured by the aptamer on the electrode, resulting in an enhanced photocurrent response. Under the optimized conditions, the present PEC sensor reveals a good linear response to Aβ peptide ranging from 1.0 fM to 100 nM with a detection limit of 0.87 fM. The present signal-on cathodic PEC bioassay possesses the potential to create a new paradigm in amplified PEC assays that could provide outstanding performance for bioanalysis.
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