生物
家蚕
细胞周期
E2F型
细胞生物学
基因
DNA复制
DNA修复
细胞周期检查点
转录因子
抄写(语言学)
遗传学
语言学
哲学
作者
Peng Chen,Ling Wang,Y. F. Long,George H. Liang,Xiu Yang,Rui Zhang,Xia Jiang,Yan Zhu,Min-Hui Pan,Cheng Lu
出处
期刊:Insect Science
[Wiley]
日期:2021-12-15
卷期号:29 (4): 1006-1016
被引量:1
标识
DOI:10.1111/1744-7917.12991
摘要
The E2F family of transcription factors is crucial for cell cycle progression and cell fate decisions. Although E2Fs have been widely studied in mammals, there have been few studies performed in insects. Here, we determined the function of E2F4 in the silkworm, Bombyx mori. We demonstrate that E2F proteins are highly conserved among species from lower animals to higher mammals. Overexpression of the BmE2F4 gene led to cell cycle arrest in the G1 phase, whereas interfering with the BmE2F4 mRNA led to accumulation of cells in the S phase. These results indicate that BmE2F4 is important in cell cycle regulation. We also demonstrate that the BmE2F4 gene is involved in DNA replication of BmN-SWU1 cells and DNA synthesis in the silk gland. Furthermore, we identified a protein called Bm14-3-3ζ that can interact with BmE2F4 and allow it to localize in the nucleus. Overexpression of the Bm14-3-3ζ gene led to cell cycle arrest in the G1 phase, while knocking down the gene increased the proportion of cells in S phase. These findings provide important insights into the function of E2F transcription factors and increase our understanding of their involvement in cell cycle regulation.
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