石墨烯
检出限
拉曼散射
拉曼光谱
适体
氧化铟锡
氧化物
材料科学
化学
纳米技术
纳米颗粒
荧光
色谱法
光学
有机化学
物理
图层(电子)
生物
遗传学
作者
Peifang Chen,Chenbiao Li,Xiaoyuan Ma,Zhouping Wang,Yin Zhang
出处
期刊:Food Control
[Elsevier]
日期:2022-04-01
卷期号:134: 108748-108748
被引量:27
标识
DOI:10.1016/j.foodcont.2021.108748
摘要
Aflatoxin B1 (AFB1) has the strongest carcinogenesis and mutagenesis effects. Herein, a novel ratiometric surface-enhanced Raman scattering (SERS) aptasensor based on graphene oxide (GO) as a corrective internal standard (IS) and signal amplifier was constructed for reliable detection of AFB1. 4-Mercaptobenzoic acid as Raman reporter embedded in [email protected] core-shell nanoparticles and AFB1aptamer conjugated to the Ag shell acted as the signal probes ([email protected] NPs-AFB1apt). AFB1apt was attached to the surface of GO with Raman signals modified Au NPs/Indium tin oxide glass (GO/Au NPs/ITO) via π–π stacking interaction, facilitating further amplification of the signal. With an increase of AFB1 concentration (0.0001 ng/mL–100 ng/mL), the probes were released owing to the affinity of aptamers, causing the decreased 4-MBA intensity (I4-MBA) and unchanged GO intensity (IGO). Therefore, the quantitative test was achieved based on the ratiometric value (I4-MBA/IGO). A good negative correlation (R2 = 0.992) was achieved and the limit of detection (LOD) was as low as 0.1 pg/mL, proving the proposed sensor can become an efficient analytical tool and shows a bright application prospect in the food detection field.
科研通智能强力驱动
Strongly Powered by AbleSci AI